黄芪葛根联用对KKA<sup>y</sup>小鼠心脏组织GRP78、CHOP和PUMA表达的影响

车宇娥; 高誉珊; 王岩飞; 张晓凤; 傅亮; 李姝玉; 冯婧; 吴丹; 史长华; 李霞; 王谦

doi:10.3969/j.issn.1006-2157.2021.02.007

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黄芪葛根联用对KKA^y小鼠心脏组织GRP78、CHOP和PUMA表达的影响

中药药理 | 更新时间：2021-04-28

- 黄芪葛根联用对KKA^y小鼠心脏组织GRP78、CHOP和PUMA表达的影响
- Effects of astragalus and puerarin on the expression of GRP78, CHOP and PUMA in heart tissues of KKA^y mice
- 北京中医药大学学报 2021年44卷第2期页码：128-133
- 作者机构：
  
  北京中医药大学　北京　 100029
- 作者简介：
  
  车宇娥，女，在读硕士生
  # 通信作者：王谦，女，博士，教授，硕士生导师，主要研究方向：中医药治疗糖尿病合并症的机制研究，E-mail：wangqianchai@163.com
- 基金信息：
  
  国家自然科学基金项目(81072926);北京高等学校青年英才计划项目(YETP0794)
- DOI：10.3969/j.issn.1006-2157.2021.02.007
  中图分类号： R285.5
- 收稿：2020-09-10，
  
  纸质出版：2021-02-28
- 稿件说明：
移动端阅览
车宇娥, 高誉珊, 王岩飞, 等. 黄芪葛根联用对KKA^y小鼠心脏组织GRP78、CHOP和PUMA表达的影响[J]. 北京中医药大学学报, 2021,44(2):128-133.

Yu’e Che, Yushan Gao, Yanfei Wang, et al. Effects of astragalus and puerarin on the expression of GRP78, CHOP and PUMA in heart tissues of KKA^y mice[J]. Journal of Beijing University of traditional Chinese Medicine, 2021, 44(2): 128-133.
车宇娥, 高誉珊, 王岩飞, 等. 黄芪葛根联用对KKA^y小鼠心脏组织GRP78、CHOP和PUMA表达的影响[J]. 北京中医药大学学报, 2021,44(2):128-133. DOI： 10.3969/j.issn.1006-2157.2021.02.007.

Yu’e Che, Yushan Gao, Yanfei Wang, et al. Effects of astragalus and puerarin on the expression of GRP78, CHOP and PUMA in heart tissues of KKA^y mice[J]. Journal of Beijing University of traditional Chinese Medicine, 2021, 44(2): 128-133. DOI： 10.3969/j.issn.1006-2157.2021.02.007.

摘要

目的

探讨黄芪注射液联合葛根素注射液通过缓解内质网应激(ERS)、抑制心肌细胞凋亡，改善2型糖尿病(T2DM)动物KKA

小鼠心脏损害，防治糖尿病心肌病的机制。

方法

11周龄的KKA

糖尿病模型小鼠按血糖随机分为模型组和黄芪葛根组(黄芪注射液3 mL/kg联合葛根素注射液1.3 mL/kg进行治疗)，同周龄雄性C57BL/6J小鼠作为正常对照组，适应性饲养1周后，于12周龄开始实验干预，在20、24和28周龄分批处理小鼠。电镜观察心肌形态改变，RT-PCR及Western Blot检测心脏组织葡萄糖调节蛋白78(GRP78)、C/EBP同源蛋白(C/EBP homologous Protein

CHOP)、p53凋亡上调基因(p53 up-regulate modulator of apoptosis

PUMA)基因和蛋白的表达。

结果

透射电镜显示，模型组小鼠心肌细胞细胞核、内质网和线粒体肿胀，黄芪葛根组较模型组情况显著改善；RT-PCR和Western Blot结果显示，与正常组相比，3个周龄段模型组GRP78的基因和蛋白表达水平均明显升高，黄芪葛根组GRP78的基因和蛋白表达较模型组均显著下降，差异具有统计学意义(

0.05)。CHOP和PUMA的基因和蛋白表达，3个周龄段模型组较正常组均增加(

0.05)，24、28周龄黄芪葛根组较模型组均下降((

0.05)。

结论

黄芪注射液与葛根素注射液联用对糖尿病小鼠心肌细胞超微结构具有保护作用，可缓解内质网应激，抑制心肌细胞凋亡，这可能是其防治糖尿病心肌病的机制之一。

Abstract

Objective

To investigate the mechanism of astragalus injection combined with puerarin injection in improving the cardiac function of type 2 diabetic(T2DM) KKA

mice by alleviating endoplasmic reticulum stress(ERS) and inhibiting cardiomyocyte apoptosis.

Methods

11-week-old KKA

diabetes model male mice were randomly divided into model group and astragalas and puerarin group (astragalus injection 3 mL/kg combined with puerarin injection 1.3 mL/kg) according to their blood sugar. Male C57BL/6J mice of the same age were used as the normal control group. After one week of adaptive feeding

experimental intervention was started at 12 weeks of age

and mice were processed in batches at 20

24 and 28 weeks of age. The morphological changes of myocardium were observed under electron microscope. RT PCR and Western Blot were used to detect the gene and protein expressions of glucose-regulated protein 78 (GRP78)

C/EBP homologous protein (CHOP)

and p53 up-regulate modulator of apoptosis (PUMA) in heart tissue.

Results

Transmission electron microscopy showed that the nuclei

endoplasmic reticulum and mitochondria of the model group were swollen; compared with that

those in the astragalus and puerarin group were significantly better. The results of Real-time PCR and Western Blot showed that compared with the normal group

the GRP78 gene and protein expression levels of the 20-

24-and 28-week-old mice in the model group were significantly higher

and the gene and protein expression of GRP78 in the astragalus and puerarin group were significantly lower than that of the model group (

0.05). The gene and protein expressions of CHOP and PUMA were increased in the 20-

24-and 28-week-old mice in the model group

compared with the normal group (

0.05)

and they were both lower in the 24-week-old and 28-week-old astragalus and puerarin groups compared with the model group (

0.05).

Conclusion

Astragalus injection combined with puerarin injection seems to have a protective effect on the ultrastructure of diabetic cardiomyocytes. The way it works may be related to alleviating endoplasmic reticulum stress and inhibiting cardiomyocyte apoptosis.

关键词

Keywords

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黄芪葛根联用对KKAy小鼠心脏组织GRP78、CHOP和PUMA表达的影响

Effects of astragalus and puerarin on the expression of GRP78, CHOP and PUMA in heart tissues of KKAy mice

DOI：10.3969/j.issn.1006-2157.2021.02.007

摘要

Abstract

关键词

Keywords

references

黄芪葛根联用对KKA^y小鼠心脏组织GRP78、CHOP和PUMA表达的影响

Effects of astragalus and puerarin on the expression of GRP78, CHOP and PUMA in heart tissues of KKA^y mice