益气通阳颗粒对免疫性血小板减少症模型小鼠的治疗作用及机制

杨秀鹏; 许勇钢; 麻柔; 杜宇; 彭勍; 王洪志; 蔡晓青; 魏丢; 李芮; 王德秀; 李柳

doi:10.3969/j.issn.1006-2157.2023.04.009

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益气通阳颗粒对免疫性血小板减少症模型小鼠的治疗作用及机制

实验研究 | 更新时间：2023-05-09

- 益气通阳颗粒对免疫性血小板减少症模型小鼠的治疗作用及机制
- Therapeutic effect and mechanism of Yiqi Tongyang Granule in treating immune thrombocytopenia model mice
- 北京中医药大学学报 2023年46卷第4期页码：502-508
- 作者机构：
  
  中国中医科学院西苑医院　北京　100091
- 作者简介：
  
  杨秀鹏，女，博士，副主任医师
  李柳，女，硕士，主任医师，硕士生导师，主要研究方向：中西医结合血液病，E-mail：liliu19662128@sina.com
- 基金信息：
  
  国家自然基金面上项目(81072928);北京市科学技术委员会“十病十药”专项课题项目(Z161100001816019)
- DOI：10.3969/j.issn.1006-2157.2023.04.009
  中图分类号： R285.5
- 纸质出版日期：2023-04-30，
  
  网络出版日期：2023-03-08，
  
  收稿日期：2022-11-18，
- 稿件说明：
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杨秀鹏, 许勇钢, 麻柔, 等. 益气通阳颗粒对免疫性血小板减少症模型小鼠的治疗作用及机制[J]. 北京中医药大学学报, 2023,46(4):502-508.

YANG Xiupeng, XU Yonggang, MA Rou, et al. Therapeutic effect and mechanism of Yiqi Tongyang Granule in treating immune thrombocytopenia model mice[J]. Journal of Beijing University of Traditional Chinese Medicine, 2023,46(4):502-508.
杨秀鹏, 许勇钢, 麻柔, 等. 益气通阳颗粒对免疫性血小板减少症模型小鼠的治疗作用及机制[J]. 北京中医药大学学报, 2023,46(4):502-508. DOI： 10.3969/j.issn.1006-2157.2023.04.009.

YANG Xiupeng, XU Yonggang, MA Rou, et al. Therapeutic effect and mechanism of Yiqi Tongyang Granule in treating immune thrombocytopenia model mice[J]. Journal of Beijing University of Traditional Chinese Medicine, 2023,46(4):502-508. DOI： 10.3969/j.issn.1006-2157.2023.04.009.

摘要

目的

探讨益气通阳颗粒对免疫性血小板减少症模型小鼠的治疗作用及作用机制。

方法

以BALB/c小鼠为研究对象，腹腔注射豚鼠抗小鼠血小板血清建立免疫性血小板减少症小鼠模型。造模小鼠随意分为模型组、益气通阳组、金薯叶组、泼尼松组，每组10只；10只未造模小鼠为对照组。各治疗组分别以益气通阳颗粒(17.4 g/kg)、酸酸泼尼松片(9.1 mg/kg)和金薯叶止血合剂(3.9 mL/kg)，灌胃11 d。检测各组小鼠的外周血血小板(PLT)、白细胞(WBC)计数和血红蛋白(Hb)含量；通过胸骨骨髓涂片和骨髓病理比较各组的骨髓巨核细胞总数，以及不同类型巨核细胞的比例；检测小鼠脾脏和胸腺的脏器系数，观察脾脏和胸腺的病理改变；流式细胞术检测小鼠淋巴细胞亚群。

结果

与对照组相比，模型组小鼠PLT计数减少(

0.05)，经益气通阳颗粒、泼尼松和金薯叶止血合剂治疗后PLT计数增加(

0.05)。与对照组相比，模型组小鼠原始和幼稚巨核细胞数量增加、产板巨核细胞比例减少(

0.05)，经益气通阳颗粒治疗后原始和幼稚巨核细胞比例减少，而产板巨核细胞比例增加(

0.05)。病理观察发现，模型组脾脏增大，可见较多髓外造血，各治疗组与模型组相比脾脏减小且髓外造血现象减少；经泼尼松治疗的免疫性血小板减少症小鼠胸腺萎缩。流式细胞术检测发现，与对照组相比，模型组小鼠细胞毒性T细胞(Tc)、辅助性T细胞(Th)、调节性T细胞(Treg)细胞百分比降低，经益气通阳颗粒治疗后升高(

0.05)。

结论

益气通阳颗粒可通过调控淋巴细胞亚群调节免疫性血小板减少症小鼠的免疫状态，通过影响骨髓产板巨核细胞的数量促进外周血PLT升高，其疗效与泼尼松相近，明显优于金薯叶止血合剂。

Abstract

Objective

To explore the therapeutic effect of

Yiqi Tongyang

Granule in the treatment of immune thrombocytopenia mice and the underlying mechanism.

Methods

BALB/c mice were treated with anti-mouse platelet serum from guinea pig to establish an immune thrombocytopenia mouse model. Model mice were randomly devided into the model group

the prednisone group

the

Yiqi Tongyang

Granule group

and the

Jinshuye

group; 10 unmodeled mice as the control group. The mice were treated with

Yiqi Tongyang

Granule(17.4 g/kg)

prednisone(9.1 mg/kg)

and

Jinshuye

Styptic Mixture(3.9 mL/kg) by gavage for 11 days

and the platelets (PLT)

white blood cells (WBC)

and hemoglobin (Hb) counts in the mice were measured. Bone marrow megakaryocyte counts were compared by bone marrow smears and bone marrow pathology

and the proportions of different types of megakaryocytes in bone marrow were analyzed. The organ coefficients and pathological changes of the spleen and thymus were observed in mice. Lymphocyte subsets were detected using a flow cytometer.

Results

Compared with the control group

the number of PLT in immune thrombocytopenia mice was reduced (

0.05)

and after treatment with

Yiqi Tongyang

Granule

prednisone

and

Jinshuye

Styptic Mixture

the number of PLT was increased (

0.05). Compared with the control group

the number of bone marrow megakaryocytes was increased and the proportion of PLT-producing megakaryocytes was decreased in immune thrombocytopenia mice (

0.05). The number of megakaryocytes was decreased after treatment with

Yiqi Tongyang

Granule (

0.05)

the ratio of primitive and naive megakaryocytes was decreased

and the proportion of PLT-producing megakaryocytes was increased in the immune thrombocytopenia mice (

0.05). The spleen was observed to be enlarged in the immune thrombocytopenia mice based on the spleen organ coefficient and pathology

and extramedullary hematopoiesis was found to be common in the spleens of immune thrombocytopenia mice. Thymus atrophy was very obvious in immune thrombocytopenia mice treated with prednisone based on the thymus organ coefficient and pathology. The percentages of Tc

and Treg cells of immune thrombocytopenia mice were reduced

and these percentages were increased after treatment with

Yiqi Tongyang

granules (

0.05).

Conclusion

Yiqi Tongyang

Granule could regulate the immune state of immune thrombocytopenia mice by regulating the lymphocyte subpopulations and increase the PLT levels in peripheral blood by affecting the number of bone marrow PLT-producing megakaryocytes. The therapeutic effect of

Yiqi Tongyang

Granule was similar to that of prednisone and clearly superior to that of

Jinshuye

Styptic Mixture.

关键词

免疫性血小板减少症益气通阳颗粒淋巴细胞亚群小鼠

Keywords

immune thrombocytopeniaYiqi Tongyang Granulelymphocyte subsetsmice

references

中华医学会血液学分会血栓与止血学组．成人原发免疫性血小板减少症诊断与治疗中国指南(2020年版)[J]. 中华血液学杂志，2020, 41(8): 617－622.

杨秀鹏，麻柔，苏根元，等.3种提取工艺制备的益气通阳汤对免疫性血小板减少小鼠作用的比较研究[J]. 中药新药与临床药理，2018, 29(1): 32－35.

杨宇飞，周霭祥，麻柔．免疫性血小板减少性紫癜动物模型的建立[J]. 中华血液学杂志，1994, 15(3): 160－161.

李柳，王德秀，吕妍，等. 益气通阳方加味治疗慢性免疫性血小板减少症临床观察[J]. 中华中医药杂志，2020, 35(1)：461－463.

WEI Y, JI XB, WANG YW, et al. High-dose dexamethasone vs prednisone for treatment of adult immune thrombocytopenia: a prospective multicenter randomized trial[J].Blood, 2016, 127(3): 296－302.

麻柔，许勇钢，杨晓红．一种用于治疗慢性或难治性免疫性血小板减少症的中药药物及中药制剂制备方法：201110289325.2[P].2013－11－27.

许勇钢，杨晓红，王洪志，等. 金薯叶止血合剂对ITP模型动物的治疗作用[J]. 中国中医基础医学杂志，2006，12(9)：674－676.

虎松艳，汪源，黄欣俊，等. 不同频率糖皮质激素大鼠骨质疏松造模对软组织和免疫系统的影响[J]. 实用医学杂志，2015，31(22)：3649－3653.

孙少馨，徐杰，周玥琲，等. 凉血解毒中药对寻常型银屑病Th1/Th2平衡的影响[J].北京中医药大学学报，2015，38(11)：767－771.

宋爱华，王昕升，李艳静．自身免疫性疾病患者免疫学指标和血清细胞因子的检测及临床意义[J].河北医药，2015，37(22)：3379－3381.

吴伯艳，吴东升，练成宇，等．原发免疫性血小板减少症患者外周血淋巴细胞亚群、NKT细胞及Th1/Th2细胞因子的变化及意义[J].广东医科大学学报，2022，40(5)：549－552.

YANG XP, MA R, YANG XH, et al. Effect of Yiqi Tongyang Decoction on blood T cell subsets in patients with chronic immune thrombocytopenia[J]. Chin J Integr Med, 2017，23(9): 706－713.

傅磊，李亮，卞建军，等．调节性T细胞及调节性B细胞在成人原发免疫性血小板减少症中的表达研究[J].临床内科杂志，2022，39(11)：758－760.

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