人参皂苷Rb<sub>1</sub>、小檗碱及其联合应用对肥胖小鼠肠道菌群的影响

叶紫梦玮; 徐冰蕊; 赵祎; 莫芳芳; 张毅; 方心; 华静; 隋芯; 高思华; 赵丹丹

doi:10.3969/j.issn.1006-2157.2023.11.009

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人参皂苷Rb₁、小檗碱及其联合应用对肥胖小鼠肠道菌群的影响

实验研究 | 更新时间：2023-12-08

- 人参皂苷Rb₁、小檗碱及其联合应用对肥胖小鼠肠道菌群的影响
- Effect of ginsenoside Rb₁, berberine, and their combination on intestinal flora in obese mice
- 北京中医药大学学报 2023年46卷第11期页码：1541-1553
- 作者机构：
  
  1.北京中医药大学中医学院糖尿病研究中心　北京　100029
  2.北京开放大学城市管理学院
  3.北京中医药大学第三附属医院
  4.北京中医药大学信息与教育技术中心
- 作者简介：
  
  叶紫梦玮，女，在读硕士生
  # 赵丹丹，女，博士，副研究员，硕士生导师，主要研究方向：脏腑相关理论防治糖尿病等代谢性疾病的基础与临床研究，E-mail：bucmzhaodandan@163.com
- 基金信息：
  
  国家自然科学基金项目(82174329);中医药传承与创新“百千万”人才工程（岐黄工程）岐黄学者项目(1040063321005)
- DOI：10.3969/j.issn.1006-2157.2023.11.009
  中图分类号： R285.5
- 纸质出版日期：2023-11-30，
  
  网络出版日期：2023-09-12，
  
  收稿日期：2023-05-19，
- 稿件说明：
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叶紫梦玮, 徐冰蕊, 赵祎, 等. 人参皂苷Rb₁、小檗碱及其联合应用对肥胖小鼠肠道菌群的影响[J]. 北京中医药大学学报, 2023,46(11):1541-1553.

YE Zimengwei, XU Bingrui, ZHAO Yi, et al. Effect of ginsenoside Rb₁, berberine, and their combination on intestinal flora in obese mice[J]. Journal of Beijing University of Traditional Chinese Medicine, 2023,46(11):1541-1553.
叶紫梦玮, 徐冰蕊, 赵祎, 等. 人参皂苷Rb₁、小檗碱及其联合应用对肥胖小鼠肠道菌群的影响[J]. 北京中医药大学学报, 2023,46(11):1541-1553. DOI： 10.3969/j.issn.1006-2157.2023.11.009.

YE Zimengwei, XU Bingrui, ZHAO Yi, et al. Effect of ginsenoside Rb₁, berberine, and their combination on intestinal flora in obese mice[J]. Journal of Beijing University of Traditional Chinese Medicine, 2023,46(11):1541-1553. DOI： 10.3969/j.issn.1006-2157.2023.11.009.

摘要

目的

探讨人参皂苷Rb

、小檗碱及其联合应用对高脂饮食诱导肥胖小鼠肠道菌群的影响。

方法

42只SPF级雄性C57BL/6J小鼠高脂饲料喂养12周建立肥胖小鼠模型。将符合肥胖标准的32只肥胖小鼠任意分为4组：对照组、人参皂苷Rb

组(20 mg/kg)、小檗碱组(50 mg/kg)、人参皂苷Rb

＋小檗碱组(人参皂苷Rb

20 mg/kg ＋小檗碱50 mg/kg)，每组8只。连续灌胃给药8周，给药期间小鼠继续给予高脂饲料喂养。每周记录各组小鼠的体质量，每2周检测小鼠空腹血糖。实验结束后留取粪便，进行16 S rDNA测序，观察各组小鼠肠道菌群变化。

结果

与对照组比较，人参皂苷Rb

组、小檗碱组和人参皂苷Rb

＋小檗碱组第4～6周小鼠体质量降低，第4、6、8周小鼠空腹血糖降低(均

0.05)。人参皂苷Rb

组、小檗碱组和人参皂苷Rb

＋小檗碱组高脂饮食诱导的肠道生态失调均改善，拟杆菌门/厚壁菌门值增加，脱铁杆菌门、小螺菌属、螺杆菌属、多尔菌属、瘤胃球菌属和臭气杆菌属等有害菌群的相对丰度降低。

结论

人参皂苷Rb

、小檗碱及其联合应用可降低高脂饮食诱导的肥胖小鼠体质量，作用机制可能与其调节肠道菌群的结构有关。

Abstract

Objective

To investigate the effect of ginsenoside Rb

berberine

and their combination on the intestinal flora in obese mice induced by a high-fat diet.

Methods

Forty-two male SPF grade C57BL/6J mice were fed on a high-fat diet for 12 consecutive weeks to establish an obese mice model. The 32 up to the standard obese mice were randomly divided into four groups: the control group

ginsenoside Rb

group (20 mg/kg)

berberine group (50 mg/kg)

and ginsenoside Rb

+ berberine group (ginsenoside Rb

20 mg/kg+ berberine 50 mg/kg)

with eight mice in each group. The mice in each group received the corresponding drugs by gavage for 8 consecutive weeks

and the mice were fed with high-fat diet during the administration period. The body weight of the mice in each group was recorded every week

and the fasting blood glucose of mice in each group was detected every 2 weeks. After the experiment

fecal samples were collected for 16 S rDNA sequencing to observe the changes in the intestinal flora in each group of mice.

Results

Compared with the control group

the body weight from 4 to 6 weeks in the ginsenoside Rb

group

the berberine group and the ginsenoside Rb

+ berberine group were reduced

and the fasting blood glucose at 4

6 and 8 weeks were reduced (

0.05). Furthermore

the imbalanced intestinal flora induced by the high-fat diet were improved

manifesting as an increased ratio of Bacteroides/Firmicutes

and the relative abundance of harmful bacteria

such as Deferribacteres

Mucispirillum

Helicobacter

Dorea

Ruminococcus

and

Odoribacter

were reduced.

Conclusion

Ginsenoside Rb

berberine

and their combination could reduce the body weight of mice with diet-induced obesity

and this effect is likely mediated through the modulation of the intestinal flora composition.

关键词

人参皂苷Rb1小檗碱肥胖肠道菌群小鼠

Keywords

ginsenoside Rb1berberineobesityintestinal floramice

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人参皂苷Rb1、小檗碱及其联合应用对肥胖小鼠肠道菌群的影响

Effect of ginsenoside Rb1, berberine, and their combination on intestinal flora in obese mice

DOI：10.3969/j.issn.1006-2157.2023.11.009

摘要

Abstract

关键词

Keywords

references

人参皂苷Rb₁、小檗碱及其联合应用对肥胖小鼠肠道菌群的影响

Effect of ginsenoside Rb₁, berberine, and their combination on intestinal flora in obese mice