防风均一多糖SP800301的化学结构研究

王欣钰; 范海涛; 何新阳; 孙萌; 姜艳艳; 刘斌

doi:10.3969/j.issn.1006-2157.2024.06.017

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防风均一多糖SP800301的化学结构研究

中药撷英 | 更新时间：2024-07-02

- 防风均一多糖SP800301的化学结构研究
- Chemical structure of a polysaccharide SP800301 from Saposhnikoviae Radix
- 北京中医药大学学报 2024年47卷第6期页码：853-862
- 作者机构：
  
  1.北京中医药大学中药学院　北京　102488
  2.北京电子科技职业学院生物工程学院
  3.国家中医药管理局“中药经典名方有效物质发现”重点研究室
- 作者简介：
  
  王欣钰，女，在读硕士生
  #刘斌，男，博士，教授，博士生导师，主要研究方向：中药复方有效成分(组分)发现与新药创制，E-mail: liubinyn67@163.com
- 基金信息：
  
  国家自然科学基金项目(82074283);中央高校基本科研业务费专项资金资助(2023-JYB-KYPT-15)
- DOI：10.3969/j.issn.1006-2157.2024.06.017
  中图分类号： R284.2
- 纸质出版日期：2024-06-30，
  
  网络出版日期：2024-05-10，
  
  收稿日期：2024-01-19，
- 稿件说明：
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王欣钰, 范海涛, 何新阳, 等. 防风均一多糖SP800301的化学结构研究[J]. 北京中医药大学学报, 2024,47(6):853-862.

WANG Xinyu, FAN Haitao, HE Xinyang, et al. Chemical structure of a polysaccharide SP800301 from Saposhnikoviae Radix[J]. Journal of Beijing University of Traditional Chinese Medicine, 2024,47(6):853-862.
王欣钰, 范海涛, 何新阳, 等. 防风均一多糖SP800301的化学结构研究[J]. 北京中医药大学学报, 2024,47(6):853-862. DOI： 10.3969/j.issn.1006-2157.2024.06.017.

WANG Xinyu, FAN Haitao, HE Xinyang, et al. Chemical structure of a polysaccharide SP800301 from Saposhnikoviae Radix[J]. Journal of Beijing University of Traditional Chinese Medicine, 2024,47(6):853-862. DOI： 10.3969/j.issn.1006-2157.2024.06.017.

摘要

目的

对从防风中分离得到的均一多糖SP800301进行结构解析，确定化学结构。

方法

采用二乙氨基乙基(DEAE)－纤维素、葡聚糖凝胶G－75等柱色谱法，分离纯化防风多糖；采用电喷雾串联质谱、气相色谱质谱联用、核磁共振等技术，对分离得到的防风均一多糖SP800301的分子量分布、单糖组成、寡糖片段、糖残基类型和糖苷键连接方式等进行分析，确定结构；采用电子显微镜和原子力显微镜扫描法表征防风均一多糖SP800301的外貌特点。

结果

防风多糖SP800301均一性良好，分子量为9.1 × 10

g/mol，糖醛酸含量52.72%，由鼠李糖、半乳糖醛酸、葡萄糖、半乳糖和阿拉伯糖组成，摩尔比为4.3∶58.1∶25.4∶5.0∶7.3。主要以聚半乳糖醛酸为主链，支链由鼠李糖、半乳糖醛酸、半乳糖、葡萄糖和阿拉伯糖组成，其中，阿拉伯糖在支链末端，糖链中部分半乳糖醛酸形成了甲酯。

结论

明确了防风中均一多糖SP800301的化学结构，丰富了防风中多糖的化学成分内涵，为进一步研究防风多糖的免疫调节作用机制奠定基础，为防风在临床的应用提供科学依据。

Abstract

Objective

To perform structural analysis on the homogeneous polysaccharide SP800301 isolated from

Saposhnikoviae Radix

to determine its chemical structure.

Methods

Polysaccharides were separated and purified from

Saposhnikoviae Radix

using column chromatography

such as DEAE-cellulose and Sephadex G-75. The molecular weight distribution

monosaccharide composition

oligosaccharide fragments

sugar residue types

and glycosidic bond connection of the isolated homogeneous polysaccharide SP800301 from

Saposhnikoviae Radix

were analyzed using electrospray ionization multistage mass spectrometry

gas chromatography-mass spectrometry

and nuclear magnetic resonance to determine its structure. The appearance characteristics of the

Saposhnikoviae Radix

polysaccharide were characterized using electron and atomic force microscopy.

Results

Polysaccharide SP800301 extracted from

Saposhnikoviae Radix

had good homogeneity

with a molecular weight of 9.1 × 10

g/mol. The uronic acid content was 52.72%. The monosaccharide group comprised rhamnose

galacturonic acid

glucose

galactose

and arabinose

with a molar ratio of 4.3: 58.1: 25.4: 5.0: 7.3. The polysaccharide was mainly composed of polygalacturonic acid as the primary chain. The branched chain was comprised of rhamnose

galacturonic acid

galactose

glucose

and arabinose

with arabinose at the end of the branched chain and part of the galacturonic acid in the sugar chain forming a methyl ester.

Conclusion

This study clarified the chemical structure of the homogeneous polysaccharide SP800301 from

Saposhnikoviae Radix

enriched the chemical composition of

Saposhnikoviae Radix

polysaccharides

laid the foundation for further research on the immune regulatory mechanism of

Saposhnikoviae Radix

polysaccharides

and provided a scientific basis for clinically using

Saposhnikoviae Radix

关键词

防风多糖化学结构半乳糖醛酸阿拉伯糖物质基础

Keywords

Saposhnikoviae Radixpolysaccharideschemical structuregalacturonic acidarabinosematerial basis

references

国家药典委员会．中华人民共和国药典：一部 [M].北京：中国医药科技出版社：2020: 156.

郭樱，杨玉峰，胡文娟，等. 乌梅防风四物汤治疗慢性荨麻疹的临床研究[J]. 河北中医药学报，2021, 36(3): 21-23.

蒋勇，钟淑欣，何升华，等. 基于网络药理学探究防风中生物活性成分及对类风湿关节炎的作用机制[J]. 中草药，2023, 54(17): 5601-5618.

常潞，荆文光，程显隆，等. 防风化学成分、药理作用研究进展及质量标志物预测分析[J]. 中国现代中药，2022, 24(10): 2026-2039.

刘华，田嘉铭，孙黎，等. 正常小鼠巨噬细胞及外周血淋巴细胞亚群对防风多糖干预的反应[J]. 中国组织工程研究与临床康复，2008，12(18): 3475-3478.

周勇，马学清，严宣佐，等. 防风多糖JBO－6体内对小鼠兔疫功能的影响及抗肿瘤作用[J]. 北京中医药大学学报，1996, 19(4): 25-27.

孙萌，范海涛，姜艳艳，等. 防风多糖体内外免疫调节活性研究[J/OL]. 中华中医药学刊，2023: 1-14. (2023-08-24). https://kns.cnki.net/kcms/detail/21.1546.R.20230824.1051.004.htmlhttps://kns.cnki.net/kcms/detail/21.1546.R.20230824.1051.004.html.

FAN HT, SUN M, LI J, et al. Structure characterization and immunomodulatory activity of a polysaccharide from Saposhnikoviae Radix[J]. Int J Biol Macromol, 2023, 233: 123502.

XIAO YH, LIU SC, SHEN MY, et al. Physicochemical, rheological and thermal properties of Mesona chinensis poly-saccharides obtained by sodium carbonate assisted and cellulase assisted extraction[J]. Int J Biol Macromol, 2019, 126: 30-36.

XIA YG, WANG TL, YU SM, et al. Structural characteristics and hepatoprotective potential of Aralia elata root bark polysaccharides and their effects on SCFAs produced by intestinal flora metabolism[J]. Carbohydr Polym, 2019, 207: 256-265.

ZHANG WJ, XIANG QF, ZHAO J, et al. Purification, structural elucidation and physicochemical properties of a polysaccharide from Abelmoschus esculentus L (okra) flowers[J]. Int J Biol Macromol, 2020，155: 740-750.

刘永玲，赵建国，赵治兵，等. PMP柱前衍生HPLC法测定八月瓜果皮多糖中单糖组成[J]. 食品研究与开发，2023: 44(21): 131-137.

黄小兰，何旭峰，杨勤，等. PMP柱前衍生化HPLC法测定地参多糖的单糖组成[J]. 食品与发酵工业，2020, 46(7): 250-256.

LEE SJ, IN G, HAN ST, et al. Structural characteristics of a red ginseng acidic polysaccharide rhamnogalacturonan I with immunostimulating activity from red ginseng[J]. J Ginseng Res, 2020, 44(4): 570-579.

DENG YJ, HUANG LX, ZHANG CH, et al. Novel polysaccharide from Chaenomeles speciosa seeds: structural characterization, α－amylase and α－glucosidase inhibitory activity evaluation[J]. Int J Biol Macromol, 2020, 153: 755-766.

程亚楠. 多花黄精多糖提取纯化、结构表征及其体外益生活性研究[D]. 南昌：南昌大学，2023.

DONG CX, LIU L, WANG CY, et al. Structural characterization of polysaccharides from Saposhnikovia divaricata and their antagonistic effects against the immunosuppression by the culture supernatants of melanoma cells on RAW264.7 macrophages[J]. Int J Biol Macromol, 2018, 113: 748-756.

MENG Y, YI L, CHEN L, et al. Purification, structure characterization and antioxidant activity of polysaccharides from Saposhnikovia divaricata[J]. Chin J Nat Med, 2019, 17(10): 792-800.

SHIMIZU N, TOMODA M, GONDA R, et al. The major pectic Arabinogalactan having activity on the reticuloendothelial system from the roots and rhizomes of Saposhnikovia divaricata[J]. Chem Pharm Bull, 1989, 37(5): 1329-1332.

GAO YY, JIANG Y, CHEN GC, et al. A sensitive and rapid UPLC-MS/MS method for determination of monosaccharides and anti-allergic effect of the polysaccharides extracted from Saposhnikoviae Radix[J]. Molecules, 2018, 23(8): 1924.

姜绍芬，伍乐芹，戴晶晶，等．防风多糖ASPSO溶液黏度及构象研究[J]. 食品工业科技，2012, 33(4): 104-107.

李江，陆蕴如，张桂燕. 防风多糖的研究[J]. 中草药，1999, 30(9): 652-653.

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